> 
NanoCELL – Comprehensive characterization and human toxicological assessment of nanocellulose along its life cycle for reliable risk assessment and safe use in environmentally friendly packaging materials
Cellulose is the most abundant organic material on earth. Nanoscale crystallites can be obtained from biomass or cellulose-containing recyclable materials (waste). Nanocellulose (NC) is extracted from renewable raw materials and is therefore part of the bioeconomy. Nanocellulose exhibits excellent barrier properties for oxygen and mineral oils. Hence, it is able to replace barrier materials made of fossil raw materials. Nanocellulose is biodegradable and offers a promising approach to reduce persistent plastic waste.
It may thus represent a potential way out of the microplastics issue in the environment in cases, where packaging waste is accidentally released into the environment and where appropriate waste collection systems are missing. In addition, several studies indicate a better compatibility of Nanocellulose with other polymers and paper than conventionally applied materials and may therefore cause fewer issues in the recycling process eventually leading to a higher quality of the recyclate.
While it is generally accepted that cellulose produced from bacteria is non-toxic, there are still significant knowledge gaps when it comes to the impact and interactions of other cellulose nano- and microstructures such as e.g. Nanocellulose crystals and Nanocellulose fibres. The toxicity of these materials strongly depends on shape and size, surface chemistry and quality of the manufacturing process (impurities). Preliminary results indicate a low dermal and oral toxicity, but are inconsistent with regard to inhalation.
NanoCell Project Plan
Against this background, NanoCELL pursues the following objectives:
1. Development of material and analysis of Nanocellulose
- Improved manufacturing strategies for Nanocellulose from different sources (i.a. pulp and (waste-)paper) and upscaling of the synthesis approach
- Development of methods for the production of Nanocellulose -enforced foils and coatings
- Evaluation of the performance of Nanocellulose -enforced foils and coatings with regard to barrier properties against oxygen and mineral oils
- Development of standardized analytical strategies from sample preparation to physico-chemical characterization of Nanocellulose in complex matrices (such as saliva, gastric acid, intestinal fluid)
- Development of quantitative analytical techniques for the characterization of Nanocellulose along its life cycle using electron microscopy and field-flow fractionation (FFF) coupled with static (MALS) and dynamic light scattering (DLS) as well refractive index (RI) detection
2. Toxicological evaluation of Nanocellulose
- Simulation and experimental verification of the potential transport of Nanocellulose across the gastrointestinal barrier (GIT) with focus on the small intestine
- Simulation of the chemical degradation and the uptake of Nanocellulose in human cells depending on particle size and further particle properties (shape, surface charge)
- Development of smart testing strategies based on novel in vitro- und in silico-approaches for the early identification and prediction of material risks related to Nanocellulose
- Investigation of the toxicological impact of Nanocellulose based on the application of newly developed cell models (GIT, lung) and chip-based high-throughput approaches
Grant Number: BMBF - 03XP0196
Duration: 01.03.2019 - 28.02.2022
Project Lead
Project Partners
https://www.ibmt.fraunhofer.de/en.html
https://www.ivv.fraunhofer.de/en.html
http://svt.wzw.tum.de/en/
https://www.uni-saarland.de/lehrstuhl/pharm-lehr.html
Associated Partners
